Monthly Archives: April 2022

ADN environnemental article police scientifique Forenseek

French gendarmes in Ukraine: their mission, victim identification

Ukraine and numerous NGOs are building a case to bring Russia before the International Criminal Court for war crimes. On April 11, 2022, a team of 18 gendarmes from the Institut de Recherche Criminelle de la Gendarmerie Nationale (IRCGN, the French National Gendarmerie’s Forensic Science Institute) traveled to Ukraine to assist their counterparts in the difficult task of disaster victim identification. AFP interviewed Major General Patrick Touron, head of the National Gendarmerie’s Judicial Division.

The French gendarmes deployed to Ukraine on Monday have as their primary mission the identification of victims and the determination of their causes of death, explained Major General Patrick Touron, commander of the Gendarmerie’s Judicial Division, to AFP. “This team of eighteen gendarmes from the IRCGN is mainly composed of victim identification specialists, supported by two ballistics experts and an explosives specialist,” the general specified. Their mission has no fixed end date, although rotations have already been planned. “As of Tuesday, they will be in Kyiv, which for now is the location of their mission,” the general added.

The IRCGN is one of the few technical units in Europe capable of deploying to an operational theater within two hours.

Major General Patrick Touron

They will be “supervised by Ukrainian security forces” and will work alongside their Ukrainian counterparts. “The IRCGN is one of the few technical units in Europe capable of deploying within two hours to an operational theater, whether it be a theater of war (as in Mali, for example) or a natural disaster (such as the December 2004 tsunami),” General Touron emphasized. In addition to a mobile laboratory dedicated to DNA analysis, the team traveled with seven vehicles, including a truck carrying twelve refrigerated mortuary chambers to preserve autopsied bodies. The unit is fully autonomous, equipped with the means to generate its own electricity, among other logistical capacities.

We are there for the victims—to help families find their loved ones, to examine the bodies, and to determine the causes of death, – insisted the General, stressing that this was a joint effort between France and Ukraine.

Subsequently, he added, “an international structure will be established as other countries send teams on-site” to take part in the identifications and in the International Criminal Court’s (ICC) investigation into the “war crimes” committed in Ukraine. On Sunday, Ukrainian judicial authorities reported that 1,222 people had been killed in the Kyiv region since the beginning of the invasion, without specifying whether they were all civilians. Images of twenty bodies in civilian clothing lying in a street in Bucha, northwest of Kyiv, have circulated worldwide. Ukrainian authorities denounced this as a “war crime” committed by the Russian army, a charge immediately denied by Moscow. On Friday, President Emmanuel Macron declared that France was in the process of “gathering evidence” of “Russian war crimes” in Ukraine and announced the deployment of French gendarmes and magistrates to the country.

ADN environnemental article police scientifique Forenseek

Tracking the invisible through environmental DNA

DNA is everywhere—in water, soil, and even in the air. Today, scientists have at their disposal analytical methods capable of capturing and sequencing this environmental DNA (eDNA).

Thanks to major advances in PCR (Polymerase Chain Reaction) and sequencing technologies, the use of environmental DNA, or eDNA, has gained considerable momentum. The most recent breakthrough in this ever-evolving field: the collection and sequencing of DNA present in the atmosphere.

Environmental DNA: a reliable and non-invasive technique.

The principle of eDNA is based on the fact that all living organisms shed DNA fragments as they move through their environment—through urine, feces, hair, scales, secretions, and more. These genetic traces accumulate in the environment: in the ocean, rivers, lakes, soil, sediments, and, as demonstrated by two studies conducted in zoological parks, even in ambient air. Such traces provide valuable information for detecting the presence or passage of species—from microorganisms such as bacteria and viruses to elusive or rare species that are difficult, if not impossible, to monitor using conventional methods. Another key advantage of eDNA is its non-invasive nature. Only a small sample of the medium inhabited by the target species is required, without any direct intervention on the organisms themselves.

From simple traces to DNA barcoding.

Once collected, water or soil samples are sent to the laboratory for analysis. The protocol, consisting of no fewer than four steps, requires the utmost rigor on the part of scientists in order to avoid the risk of contamination.

The first step involves isolating DNA molecules using molecular biology techniques (such as precipitation and centrifugation), which are now well mastered and largely automated, thereby reducing costs. In a second step, this DNA is amplified through PCR to obtain sufficient quantities for sequencing.

DNA sequencing makes it possible to determine the sequence corresponding to a given species—that is, the specific fragment of nucleotides that characterizes and differentiates it from others. These sequences are then catalogued in international databases according to the concept of molecular barcoding, developed in 2003 by Canadian zoologist and ecologist Paul Hebert. Stored in the form of barcodes and integrated into computerized systems, DNA fragments can thus be easily compared and identified in record time.

A vast field of applications.

Within just a few years, environmental DNA (eDNA) has become a particularly valued tool for biodiversity studies. By analyzing environmental samples, researchers can identify and quantify organisms present in natural habitats, monitor rare or endangered species, or, conversely, track the emergence of invasive alien species that may disrupt ecosystems. Even more striking, the analysis of a single honey sample can reveal all the plant species visited by bees, while DNA trapped in just a few grams of sediment can provide insights into which species inhabited specific regions thousands of years ago.

Most recently, this technique has made a significant contribution to the fight against the Covid-19 pandemic. In this case, viral RNA was detected in wastewater, allowing researchers to quantify the presence of the virus and, consequently, to better anticipate the progression of the epidemic. In the future, this tool could also be used to identify the emergence of new variants at an early stage—pending further applications, equally fascinating, in many other fields.

Sources :

https://planet-vie.ens.fr/thematiques/ecologie/l-adn-environnemental-un-nouvel-outil-pour-espionner-les-especes-sauvages
https://www.ofb.gouv.fr/actualites/ladn-environnemental
https://www.notre-planete.info/actualites/4354-ADN-environnemental-biodiversite
https://www.lemonde.fr/sciences/article/2022/03/16/detecter-l-arn-environnemental-pour-suivre-l-epidemie-de-covid-19_6117759_1650684.html
https://www.encyclopedie-environnement.org/vivant/metabarcoding-codes-barres-adn-caracteriser-biodiversite/

There is no such thing as absolute proof.

The path that leads from the criminal act to its perpetrator is what we call evidence. In various forms, it is the daily concern of investigators, prosecutors, and judges. Its essentially retrospective nature makes it difficult, unpredictable, and uncertain. One cannot simply turn back time. Years of experience at the Assize Court have taught me that the path separating the most spectacular piece of evidence from a declaration of guilt is a mysterious one: just when you think you have it, it slips away; it may seem obscure, then suddenly becomes compelling. “You who enter here, beware: absolute proof does not exist” could well be the warning engraved above the doors of forensic laboratories. And rightly so.

When it’s too good to be true

He had just killed the elderly lover who had refused to comply with his financial demands when, seized by remorse, he alerted the police in the hope that they might provide the medical care which, he thought, could save him. Without revealing his identity, he fled before the emergency services arrived. Arrested shortly thereafter and confronted with the recording of his call, he admitted to the facts. However, part of the procedure containing his confession was annulled. When the investigation resumed, he chose to change his line of defense and denied the facts, a position he maintained until trial. The presiding judge allowed him ample time during his personality examination so that everyone could become familiar with the sound of his voice. Then came the review of the facts and the playing of his recorded message by the police.

During the adjournment—which is a time for relaxation and informal exchanges—all the judges and jurors recognized the accused’s voice without hesitation. The case seemed settled. Except that…

When the session resumed, and to drive the point home, the prosecution requested that the incriminating message be played again, which was done. At the next adjournment, however, one of the jurors began to have doubts. At the request of the civil party, the message was played again, but after the following recess, three jurors were questioning it. Needless to say, when asked one last time to order the message to be replayed, the presiding judge, now wise with experience, refused. The accused, whose retracted confession was known only to the professionals, might well have been acquitted if the playback of his call continued indefinitely.

The more conclusive a piece of evidence appears, the more cautious one should be

His very distinctive haircut at the time made him recognizable among a thousand: shaved all around the head, leaving only an oval brush of thick black hair on his high, slender forehead. None of these details escaped the video surveillance cameras in the underground car park he had entered barefaced to commit his crime. He was acquitted.

What better proof than to see the victim herself designate her killer with her own blood—moreover, by making a spelling mistake she habitually committed? And yet, the conviction of the accused did not prevent the movement which, invoking a miscarriage of justice, ultimately led to his pardon.

“What are the chances of a mistake in the identification of this DNA profile?” experts are frequently asked. “About one in a billion,” they reply. Until the day it becomes apparent—as I once witnessed—that a material error in an expertise hastily dictated had falsely designated the accused.

Examples of this kind abound, and one may draw a first lesson from them: the more conclusive a piece of evidence appears, the more cautious one should be.

To make progress over and over again

To make progress over and over again

None of this should discourage technicians and experts from constantly striving to refine investigative techniques, and it must be acknowledged that since the discovery of dactyloscopy, the progress made in identifying criminals or exonerating suspects has been remarkable.

Scientific research is continuous, and we can hardly imagine what future advances will further shed light on the truth.

Yet, however far progress may take us, there remains a discontinuity that will always separate the administration of evidence from the declaration of guilt, and which will sometimes frustrate even the finest investigators: that of the work of reason.

Without reproducing in full the address that the presiding judge delivers to the jurors at the end of the trial, before the court withdraws to the deliberation chamber, it is worth quoting a passage contained in what is arguably the most beautiful article of the French Penal Code—Article 353, which establishes the freedom of evidence and the principle of intimate conviction: “The law requires the judges to seek in their conscience what impression the evidence presented against the accused and the arguments for his defense have made upon their reason.” However perfect the evidence may be, its demonstration will always demand an exercise of reason, without which no finding of guilt can be pronounced. And there is nothing more uncertain than reason, even if collegiality greatly reduces its unpredictability.


The challenges of deliberation

It is, for example, particularly difficult to rule on homicidal intent. What does it mean to “intentionally cause death”? Must one probe the exact thoughts of the accused at the precise moment of an act to which he may not even have given a thought an instant earlier, and which he would regret as soon as it was committed? Did he truly intend to cause death? Quite clearly, that is impossible. It must therefore be determined whether death was the logically foreseeable material consequence of an act committed by a conscious and lucid individual.

Similarly, DNA traces found on the complainant’s underwear in a rape case, however categorical they may be, will never dispense with the need to question consent; nor will defense injuries observed on her wrists relieve the court from inquiring into the origins and circumstances of the struggle.

Not to forget—and this may be the essential point—that a trial, where doubt benefits the accused, is the singular exercise that requires judges to determine what they are certain of, in relation to facts to which they were not witnesses.

Back to the basics

Investigators and experts are, of course, fully aware of all this. But to this retrospective approach, which is their daily lot, there succeeds another—this time prospective—which is precisely the mission of that interface known as the Public Prosecutor’s Office. Its role is to assess, with all possible caution given the uncertainty that characterizes the judges’ task, and to guarantee the quality of the evidence that the prosecuting authority will submit to them.

In this uncertain—and, to be truthful, rather vertiginous—procedural chain which, beginning with the initial finding of the crime, must lead with sufficient certainty to the identification of the criminal and the punishment of the offense, it is essential that each actor be fully conscious of his or her own role and of the place he or she occupies.